I took a water sample of my very old sea monkey colony (around 2 or 3 years old) and did NOT add any of my sea monkeys to the mixture (as that would be murder). I remember reading on the packaging that the water would become dirty over time, but it was due to beneficial materials in the water, so I thought this would be a really interesting space to sample from. I’m not sure what to expect, but there must be SOMETHING in there.